“Easy Lab” methods for sensitive molecular detection of ASFV


African swine fever (ASF) is a notifiable, contagious viral hemorrhagic disease of swine, responsible for high mortality and serious economic losses. In this paper, researchers from the Friedrich-Loeffler-Institut (FLI) aimed to simplify and standardize the molecular diagnostic workflow in the lab using prefilled, ready-to-use IndiMag Pathogen Cartridges for nucleic acid extraction prior to (real-time) PCR.

The study concluded that using prefilled cartridges for DNA extraction does not reduce diagnostic sensitivity. Indeed, minimization of manual handling and increased hands-off time delivered by prefilled IndiMag Pathogen Cartridges for extraction and qPCR can reduce the risk of false-negative and -positive results, especially in high-throughput scenarios.